Plasmid transfer within and between serologically distinct strains of Rhizobium japonicum, using antibiotic resistance mutants and auxotrophs

J Bacteriol. 1981 Feb;145(2):1025-30. doi: 10.1128/jb.145.2.1025-1030.1981.

Abstract

Methionine-requiring and pantothenic acid-requiring auxotrophs of Rhizobium japanicum USDA 31, as well as highly antibiotic-resistant mutants of R. japonicum strains USDA 31, USDA 110, USDA 138, and Webster 48, were isolated. These mutants were used to transfer the P-1 group plasmids R68.45 and RP4 within and between strains USDA 31, USDA 110, and Webster 48. Attempts to demonstrate transfer of either plasmid to strain USDA 138 were unsuccessful.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Ampicillin / pharmacology
  • Escherichia coli / genetics
  • Kanamycin / pharmacology
  • Plasmids*
  • R Factors
  • Rhizobium / genetics*
  • Streptomycin / pharmacology
  • Tetracycline / pharmacology
  • Transduction, Genetic
  • Transformation, Bacterial

Substances

  • Kanamycin
  • Ampicillin
  • Tetracycline
  • Streptomycin