Activity and isoenzyme pattern of lactate dehydrogenase in neurons and astroblasts cultured from brains of chick embryos

J Neurochem. 1981 Jan;36(1):77-81. doi: 10.1111/j.1471-4159.1981.tb02379.x.

Abstract

Primary cultures of neurons and glial cells (astroblasts) prepared from brains of 8-day-old and 15-day-old chick embryos, respectively, were grown for periods between 3 and 19 days. Specific activity of lactate dehydrogenase (LDH) increased in both types of cultures as a function of time and was always significantly higher in glial cells than in neurons. Glial cell extracts were found to contain predominantly the anaerobic isoenzymatic form of LDH (LDH-M4), and this pattern did not change over a period of 19 days. Cultured neurons contained predominantly the aerobic isoenzymatic form LDH-H4, and there was a progressive appearance of all other isoenzymes over an 8-day period. These results support the hypothesis of a different energy metabolism in neurons and glia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Astrocytes / enzymology*
  • Brain / embryology*
  • Brain / enzymology
  • Cells, Cultured
  • Chick Embryo
  • Electrophoresis, Polyacrylamide Gel
  • Isoenzymes
  • L-Lactate Dehydrogenase / analysis*
  • Microscopy, Phase-Contrast
  • Neurons / enzymology*
  • Time Factors

Substances

  • Isoenzymes
  • L-Lactate Dehydrogenase