Studies of ribosomal proteins of yeast species and their hybrids: gel electrophoresis and immunochemical cross-reactions

Mol Gen Genet. 1980;179(2):273-82. doi: 10.1007/BF00425454.


The cytoplasmic ribosomal proteins (r-proteins) of seventeen yeast species of the genera Saccharomyces and Kluyveromyces were analyzed by one-dimensional gradient polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. The electrophoretic patterns of cytoplasmic r-proteins from different species display extensive differences in both the 40S and the 60S subunit. Relatedness of species suggested by r-protein patterns correlates well with that based on DNA/DNA homology (Bicknell and Douglas 1970). Immunochemical cross-reactions and antibiotic susceptibility tests were also used to compare different species. Analyses of r-proteins from two different interspecific hybrids showed that their ribosomes were hybrid, containing r-proteins from both parents. These findings are discussed in relation to the evolution of yeast species and the regulation of expression of r-proteins in eucaryotes.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Ascomycota / metabolism
  • Cross Reactions
  • Drug Resistance, Microbial
  • Electrophoresis, Polyacrylamide Gel
  • Fungal Proteins / immunology
  • Fungal Proteins / metabolism*
  • Hybridization, Genetic
  • Ribosomal Proteins / immunology
  • Ribosomal Proteins / metabolism*
  • Ribosomes / drug effects
  • Saccharomyces cerevisiae / metabolism
  • Species Specificity
  • Yeasts / metabolism*


  • Anti-Bacterial Agents
  • Fungal Proteins
  • Ribosomal Proteins