Nalidixic acid, oxolinic acid, and novobiocin inhibit yeast glycyl- and leucyl-transfer RNA synthetases

Science. 1981 Jul 24;213(4506):455-6. doi: 10.1126/science.7017932.


Nalidixic acid and novobiocin inhibit the aminoacylation and pyrophosphate exchange activities of glycyl- and leucyl-transfer RNA synthetases from bakers' yeast. Similar types of inhibition are observed for both enzymes, suggesting similar mechanisms. The potency of these inhibitors is comparable to that observed for their inhibition of in vivo DNA synthesis in eukaryotic cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acyl-tRNA Synthetases / antagonists & inhibitors*
  • Glycine-tRNA Ligase / antagonists & inhibitors*
  • Kinetics
  • Leucine-tRNA Ligase / antagonists & inhibitors*
  • Nalidixic Acid / pharmacology*
  • Novobiocin / pharmacology*
  • Oxolinic Acid / pharmacology*
  • Saccharomyces cerevisiae / enzymology*


  • Novobiocin
  • Nalidixic Acid
  • Amino Acyl-tRNA Synthetases
  • Glycine-tRNA Ligase
  • Leucine-tRNA Ligase
  • Oxolinic Acid