Modified granulocyte test for determination of mannosidosis genotype of cattle

Res Vet Sci. 1981 May;30(3):281-3.

Abstract

Recovery of alpha-mannosidase, beta-N-acetylglucosaminidase and arylsulphatase activity from granulocytes is increased by inclusion of 154 mM sodium chloride, in place of 1 mM zinc chloride, in aqueous Triton X-100 used to extract the cells. Calculation of the ratio of alpha-mannosidase to beta-N-acetylglucosaminidase and to arylsulphatase permits diagnosis of the mannosidosis genotype without recourse to examination of smears of the granulocyte preparations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylglucosaminidase / blood
  • Animals
  • Arylsulfatases / blood
  • Carbohydrate Metabolism, Inborn Errors / diagnosis
  • Carbohydrate Metabolism, Inborn Errors / veterinary*
  • Cattle
  • Cattle Diseases / diagnosis*
  • Chlorides*
  • Clinical Enzyme Tests / veterinary*
  • Granulocytes / enzymology*
  • Sodium Chloride / pharmacology
  • Zinc / pharmacology
  • Zinc Compounds*
  • alpha-Glucosidases / blood

Substances

  • Chlorides
  • Zinc Compounds
  • Sodium Chloride
  • zinc chloride
  • Arylsulfatases
  • alpha-Glucosidases
  • Acetylglucosaminidase
  • Zinc