Properties of Reference Escherichia Coli Endotoxin and Its Phthalylated Derivative in Humans

J Infect Dis. 1981 Oct;144(4):329-36. doi: 10.1093/infdis/144.4.329.

Abstract

The properties of a reference bacterial endotoxin prepared from Escherichia coli and its phthalylated derivative were studied in normal human volunteers infected intravenously with the compounds. The minimal pyrogenic dose of the reference endotoxin is about 0.1-0.5 ng/kg. The increase in white blood cell count, absolute granulocyte count, absolute immature granulocyte count, and concentrations of serum amyloid A, cortisol, and growth hormone was directly related to the concentration of reference endotoxin administered. Phthalylated reference endotoxin up to 1,000 ng/kg (at least 500 ng of the patent compound/kg) was administered to normal human volunteers without significant changes in temperature, white blood cell count, absolute granulocyte count, and concentrations of serum amyloid A, cortisol, and growth hormone. Thus, this study defines biologic properties of the new reference bacterial endotoxin in humans and demonstrates effective detoxification by phthalylation of the present compound.

Publication types

  • Clinical Trial
  • Controlled Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Endotoxins / administration & dosage
  • Endotoxins / standards*
  • Escherichia coli*
  • Female
  • Fever / chemically induced
  • Growth Hormone / blood
  • Humans
  • Hydrocortisone / blood
  • Leukocyte Count
  • Lipopolysaccharides / administration & dosage
  • Lipopolysaccharides / standards
  • Male
  • Phthalic Acids / administration & dosage
  • Phthalic Acids / standards
  • Serum Amyloid A Protein / analysis

Substances

  • Endotoxins
  • Lipopolysaccharides
  • Phthalic Acids
  • Serum Amyloid A Protein
  • Growth Hormone
  • Hydrocortisone