Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28

Hum Immunol. 1981 Dec;3(4):277-99. doi: 10.1016/0198-8859(81)90065-3.


The purification and properties of a cytotoxic mouse monoclonal anti-HLA-A2 antibody are described. This antibody, BB7.2, can be used as an HLA-A2 typing reagent with little modification of current typing techniques. It also recognizes a low frequency variant of HLA-A28. BB7.2 provides an example of an antibody which requires bivalent attachment to a polymeric antigen, e.g., a cell, to produce a readily detectable complex. This is due to a high rate of dissociation of the complex formed between a single BB7.2 combining sites and HLA-A2. The consequences of this property were investigated and some complications for potential uses of such monoclonal antibodies are discussed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / genetics
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / isolation & purification*
  • Antibody Specificity*
  • Antibody-Producing Cells / immunology
  • Binding Sites, Antibody
  • Binding, Competitive
  • Carcinoma, Ehrlich Tumor / immunology
  • Chemical Fractionation
  • Cytotoxicity, Immunologic*
  • Electrophoresis, Polyacrylamide Gel
  • Genetic Variation
  • HLA Antigens / genetics
  • HLA Antigens / immunology*
  • Humans
  • Immunoglobulin Fab Fragments
  • Immunoglobulin G / isolation & purification
  • Kinetics
  • Mice
  • Mice, Inbred BALB C
  • Peptide Hydrolases / pharmacology
  • Rabbits


  • Antibodies, Monoclonal
  • HLA Antigens
  • Immunoglobulin Fab Fragments
  • Immunoglobulin G
  • Peptide Hydrolases