The evaluation of limiting dilution assays

J Immunol Methods. 1982 Mar 12;49(2):R11-23. doi: 10.1016/0022-1759(82)90269-1.


Theoretical and practical aspects of limiting dilution assays are considered, and the maximum likelihood solution is illustrated by two worked examples. These examples show up the inferiority of the conventional regression analysis, while the advantages of the maximum likelihood over minimum chi-square methods are covered in the discussion. A program, which will perform all the necessary calculations on a pocket calculator, is listed in the Appendix.

Publication types

  • Review

MeSH terms

  • Cells, Cultured
  • Computers
  • Cytotoxicity Tests, Immunologic / methods
  • Dose-Response Relationship, Immunologic
  • Immunologic Techniques*
  • Mathematics
  • Probability
  • Regression Analysis