Doxorubicin cytotoxicity enhanced by local anesthetics in a human melanoma cell line

Cancer Treat Rep. 1982 Jan;66(1):121-5.


Local anesthetics may influence cell membrane structure and permeability. An established human melanoma cell line (SHG) derived from malignant ascites was used to evaluate the growth-inhibitory effects of local anesthetics (procaine and lidocaine) as well as of doxorubicin at incubation temperatures of 37.5 degrees C and 40 degrees C. In this system, procaine and lidocaine inhibited growth at 0.82 mg/ml and doxorubicin inhibited growth at 28 ng/ml. Noninhibitory concentrations of procaine (0.64 mg/ml) when combined with noninhibitory concentrations of doxorubicin (10 ng/ml) resulted in marked growth inhibition. Incubating temperatures of 40 degrees C enhanced all effects of procaine and doxorubicin on cell growth. These results suggest that clinically achievable concentrations of local anesthetics enhance doxorubicin cytotoxicity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cell Survival / drug effects
  • Doxorubicin / toxicity*
  • Drug Synergism
  • Humans
  • Lidocaine / pharmacology*
  • Melanoma
  • Procaine / pharmacology*
  • Temperature


  • Procaine
  • Doxorubicin
  • Lidocaine