Alterations in opsonophagocytic killing by neutrophils of Bacteroides fragilis associated with animal and laboratory passage: effect of capsular polysaccharide

J Infect Dis. 1982 Jan;145(1):72-7. doi: 10.1093/infdis/145.1.72.

Abstract

Electron microscopic studies of laboratory-passaged and animal-passaged isolates of Bacteroides fragilis showed significantly more capsular material around the latter. This observation correlated with increased survival of animal-passaged bacteria in rabbit intraperitoneal chambers and increased resistance to phagocytosis and opsonophagocytic killing by neutrophils. With an initial inoculum of 2.5 x 10(6) colony-forming units/ml, the number of bacteria surviving after incubation for 2 hr with neutrophils and pooled normal human serum was significantly (P less than 0.01) greater for animal-passaged than for laboratory-passaged bacteria. Neutrophil uptake of 14C-labeled animal-passaged and laboratory-passaged bacteria after incubation for 20 min was 45% and 63%, respectively (P less than 0.05). No significant difference in survival was found between animal-passaged and laboratory-passaged isolates of Bacteroides thetaiotaomicron. These findings suggest that on mechanism whereby the capsule of B. fragilis contributes to virulence is by inhibiting opsonophagocytic killing.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bacteroides Infections / immunology
  • Bacteroides fragilis / immunology*
  • Bacteroides fragilis / pathogenicity
  • Bacteroides fragilis / ultrastructure
  • Blood Bactericidal Activity*
  • Ferritins
  • Humans
  • Neutrophils / immunology
  • Opsonin Proteins / immunology*
  • Phagocytosis*
  • Polysaccharides, Bacterial / immunology*
  • Rabbits
  • Virulence

Substances

  • Opsonin Proteins
  • Polysaccharides, Bacterial
  • Ferritins