A third DNA polymerase from Spiroplasma citri and two other spiroplasmas

J Bacteriol. 1982 Mar;149(3):1138-41. doi: 10.1128/jb.149.3.1138-1141.1982.

Abstract

Recently, two DNA polymerases (ScA and ScB) were isolated and characterized from Spiroplasma citri. We now have found a third DNA polymerase (ScC) not only in S. citri but also in the serologically related honeybee spiroplasma BC3 and the unrelated flower spiroplasma BNR1. Enzyme ScC is N-ethylmaleimide (NEM) sensitive. The three DNA polymerases from the honeybee spiroplasma seem to be similar to the respective enzymes of S. citri. However, whereas the NEM-resistant enzyme ScA from S. citri and that from the BC3 honeybee spiroplasma are retained on DEAE-cellulose and require 0.09 M KCl for elution, the NEM-resistant enzyme A from the flower spiroplasma BNR1 is not retained.

MeSH terms

  • Chromatography, DEAE-Cellulose
  • DNA-Directed DNA Polymerase / isolation & purification*
  • DNA-Directed DNA Polymerase / metabolism
  • Ethylmaleimide / pharmacology
  • Spiroplasma / enzymology*

Substances

  • DNA-Directed DNA Polymerase
  • Ethylmaleimide