Effects of RNA and ribonuclease on the binding of estrogen and glucocorticoid receptors from MCF-7 cells to DNA-cellulose

Abstract

Effects of RNA and ribonucleases on the binding of estrogen- and glucocorticoid-receptor complexes from MCF-7 cells to calf thymus DNA-cellulose have been studied under cell-free conditions. Exogenous ribonucleases can increase binding of estrogen- and glucocorticoid-receptor complexes to DNA-cellulose. Purified RNA derived from MCF-7 cells can inhibit estrogen- and glucocorticoid-receptor binding to DNA-cellulose. Under low salt conditions, RNA appears to be associated with estrogen-receptor complexes from MCF-7 cells. These observations taken together suggest that there is an interaction between steroid hormone-receptor complexes and RNA. The implications of this interaction are discussed.