Development of hepatic lesions in male Fischer-344 rats fed AIN-76A purified diet

Toxicol Appl Pharmacol. 1982 Jan;62(1):111-20. doi: 10.1016/0041-008x(82)90107-7.


The suitability of the AIN-76A diet for Fischer-344 rats was investigated. This diet, proposed by the American Institute of Nutrition for use when a purified diet composed of refined ingredients and added vitamins and minerals is required, was tested in Sprague-Dawley rats. Male weanling Fischer-344 rats were fed three different lots of the AIN-76A diet from two suppliers. Increase in body weights and food consumption were compared to animals fed a cereal-based control diet. Animals were sacrificed at various intervals and tissues were taken for histopathological observation. By 8 weeks moderate to marked periportal lipidosis developed in livers of all rats fed the AIN-76A diet. Liver-body weight ratios over the 8-week period were significantly higher in rats fed AIN-76A diets compared to rats fed the control diet. However, growth rates of rats fed the AIN-76A diet were similar to growth rates of controls. Some rats fed the AIN-76A diet developed severe hemorrhagic lesions. The AIN-76A diet in its present form is not suitable for use with male Fischer-344 rats.

MeSH terms

  • Animal Feed / toxicity*
  • Animals
  • Body Weight
  • Food, Formulated / toxicity*
  • Hemorrhage / etiology
  • Lipidoses
  • Liver / drug effects
  • Liver / metabolism
  • Liver / pathology*
  • Male
  • Rats
  • Rats, Inbred F344