Estimation of the saturation of serum transferrin by an electrophoretic technique

Ann Clin Biochem. 1982 Jan;19(Pt 1):57-9. doi: 10.1177/000456328201900112.


An electrophoretic method is described for estimating the saturation of serum transferrin. After first precipitating most of the other proteins with Rivanol, the apo-, mono-, and di-ferric transferrins are separated by electrophoresis in urea-polyacrylamide gel. Densitometric scanning enables the percentage saturation to be calculated.

MeSH terms

  • Antibodies / analysis
  • Densitometry
  • Electrophoresis, Polyacrylamide Gel / methods
  • Humans
  • Iron / blood
  • Protein Binding
  • Transferrin / analysis*


  • Antibodies
  • Transferrin
  • Iron