A quantitative cytotoxicity assay using whole blood: application to H-2 tissue typing

J Immunol Methods. 1982 Mar 26;49(3):293-303. doi: 10.1016/0022-1759(82)90129-6.


A fluorochromatic cytotoxicity assay featuring direct use of whole blood and quantitative evaluation is described. Its application to tissue typing for H-2 antigens together with data indicating reproducibility specificity of the assay are presented. Procedures for visual and automated quantification are included.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blood Cells*
  • Cytotoxicity Tests, Immunologic / methods
  • Flow Cytometry
  • H-2 Antigens*
  • Histocompatibility Testing*
  • Immune Sera / pharmacology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred Strains


  • H-2 Antigens
  • Immune Sera