Effect of dietary retinyl acetate, beta-carotene and retinoic acid on wound healing in rats

J Nutr. 1982 Aug;112(8):1555-64. doi: 10.1093/jn/112.8.1555.


The effects of feeding various levels and combinations of retinyl acetate, beta-carotene, or retinoic acid on skin wound healing in rats was investigated. Weanling male Sprague-Dawley rats were fed a vitamin A-free diet for 2 weeks to produce marginal vitamin A status. After a paravertebral incision was made and closed with suture, one of several diets were fed for either 5 or 14 days. Surgery and recovery did not reduce liver vitamin A nor serum retinol levels compared to nonoperated pair-fed controls. Supplemental retinyl acetate feeding at five times the NRC-suggested allowance resulted in a mild, but significantly increased postmortem wound tensile strength after 5 days compared to rats fed the suggested allowance. Although a low level of retinoic acid in the diet (1.3 microgram/g diet) depressed wound strength at 5 days, a higher level (5.2 microgram/g) increased the strength 57% above controls. Still higher levels (49.1 microgram/g) did not further increase the tensile strength of the wound. beta-Carotene fed the requirement level for 5 days (with compensation made for utilization as one-sixth that of retinol) doubled wound strength compared to rats fed the requirements as retinyl acetate. Vitamin A feeding did not enhance wound strength after 14 days of feeding. It is concluded that supplemental retinyl acetate, beta-carotene, or in some cases all-trans-retinoic acid can be effective in enhancing wound strength, 5 days, but not 14 days after surgery, of young male rats with marginal vitamin A status.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Administration, Oral
  • Animals
  • Carotenoids / administration & dosage
  • Carotenoids / therapeutic use*
  • Diterpenes
  • Dose-Response Relationship, Drug
  • Male
  • Rats
  • Rats, Inbred Strains
  • Retinyl Esters
  • Skin / injuries*
  • Time Factors
  • Tretinoin / administration & dosage
  • Tretinoin / therapeutic use*
  • Vitamin A / administration & dosage
  • Vitamin A / analogs & derivatives*
  • Vitamin A / therapeutic use
  • Wound Healing / drug effects*
  • beta Carotene


  • Diterpenes
  • Retinyl Esters
  • beta Carotene
  • Vitamin A
  • Carotenoids
  • retinol acetate
  • Tretinoin