Quantitative immunoassays for diagnosis and carrier detection in cystic fibrosis

Clin Genet. 1982 May;21(5):336-41. doi: 10.1111/j.1399-0004.1982.tb01380.x.


Quantitative immunoprecipitation and immunoradiometric assays have been developed for a protein present in the serum of cystic fibrosis homozygotes, and to a lesser extent in the serum of heterozygotes. When tested on a panel of sera from 14 cystic fibrosis patients, 29 heterozygotes and 23 controls, the immunoprecipitation assay allowed correct assignments to be made on 94% of occasions with one batch of antiserum and 95% with another. With the same panel of sera, the immunoradiometric assay allowed 94% correct assignments. It is suggested that such accuracy is the maximum that can be expected in the present state of knowledge of cystic fibrosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Proteins / genetics*
  • Cystic Fibrosis / diagnosis*
  • Cystic Fibrosis / genetics
  • Genetic Carrier Screening
  • Homozygote
  • Humans
  • Immunoelectrophoresis
  • Radioimmunoassay
  • Reference Values


  • Blood Proteins