Abstract
A multisubstrate analog, formed by joining coenzyme A with spermidine through an acetic acid linkage, serves as a strong inhibitor (Ki less than 10(-8) M) of the acetylation of spermidine and histones by histone acetylase purified from calf thymus. In free solutions, this analog inhibited acetylation of the various nuclear histones to a similar extent. In isolated nuclei, this analog was found to inhibit acetylation of histones H2a and H2b very much more strongly than that of histones H3 and H4.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetylation
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Acetyltransferases / antagonists & inhibitors
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Animals
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Cattle
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Histone Acetyltransferases
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Histones / metabolism*
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Kinetics
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Saccharomyces cerevisiae Proteins*
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Spermidine / analogs & derivatives*
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Spermidine / metabolism
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Spermidine / pharmacology
Substances
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Histones
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Saccharomyces cerevisiae Proteins
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N-(2-(S-coenzyme A)acetyl)spermidine
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Acetyltransferases
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Histone Acetyltransferases
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Spermidine