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Comparative Study
, 37 (10), 3663-7

Comparative Chemical Structures of Human Alpha-Fetoproteins From Fetal Serum and From Ascites Fluid of a Patient With Hepatoma

  • PMID: 71198
Comparative Study

Comparative Chemical Structures of Human Alpha-Fetoproteins From Fetal Serum and From Ascites Fluid of a Patient With Hepatoma

Y Aoyagi et al. Cancer Res.

Abstract

Human alpha-fetoproteins were purified from umbilical cord serum and from ascites fluid of a patient with hepatoma by affinity chromatography, and their chemical compositions and terminal sequences were compared. The amino acid compositions of these alpha-fetoproteins were similar and in good agreement with the values reported by other investigators. The COOH-terminal 5-amino acid sequence determined by carboxypeptidase digestion and the NH2-terminal 20-amino acid sequence determined by an automated sequence analyzer revealed that both alpha-fetoproteins had the same terminal sequences of amino acids. The sequence analysis showed that a part of each of the proteins lacked its NH2-terminal residues for one or three amino acids. A small difference in the carbohydrate composition of each alpha-fetoprotein was observed. It was concluded that alpha-fetoproteins from fetal serum and from ascites fluid of a patient with hepatoma had very similar structures.

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