Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication

Science. 1982 Oct 29;218(4571):474-5. doi: 10.1126/science.7123245.


Monoclonal antibodies specific for 5-bromodeoxyuridine have been produced and applied in detecting low levels of DNA replication on a cell-by-cell basis in vitro. The immunoglobulin-producing hybridomas were derived from spleen cells of mice immunized with a conjugate of iodouridine and ovalbumin. The cells were fused with the plasmacytoma line SP2/0Ag14. The antibodies produced are highly specific for bromodeoxyuridine and iododeoxyuridine and do not cross-react with thymidine. DNA synthesis in cultured cells exposed to bromodeoxyuridine for as short a time as 6 minutes can be detected easily and rapidly by an immunofluorescent staining method and quantitated by flow cytometry.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Bromodeoxyuridine / analysis*
  • Bromodeoxyuridine / immunology
  • Cells, Cultured
  • DNA Replication*
  • Flow Cytometry
  • Idoxuridine / analysis*
  • Idoxuridine / immunology
  • Mice


  • Antibodies, Monoclonal
  • Bromodeoxyuridine
  • Idoxuridine