A new device for the determination of microsomal cytochrome P-450 in renal tissue preparations from various species contaminated with mitochondria and hemoglobin

Jpn J Pharmacol. 1982 Aug;32(4):679-88. doi: 10.1254/jjp.32.679.

Abstract

Using a spectrophotometer connected to a microcomputer, the carbon monoxide difference spectrum of renal microsomal dithionite-reduced cytochrome P-450 was measured avoiding the effects of the contaminating mitochondrial cytochromes and hemoglobin by subtracting the CO difference spectrum of a succinate-treated microsomal suspension from that of a dithionite-treated one. By this method, we quantitatively determined the microsomal cytochrome P-450 in kidneys from various species. The absorption peak of the renal microsomal cytochrome P-450 was about 452 nm in rats and about 450 nm in mice, hamsters, rabbits, guinea pigs, dogs, and pigs. Compared with the renal cytochrome P-450 contents in rats, the contents on a g tissue basis were greater in pigs, dogs, and mice and were about the same in hamsters and rabbits. The renal cytochrome P-450 contents in guinea pigs were less than those in rats.

MeSH terms

  • Animals
  • Carbon Monoxide
  • Cricetinae
  • Cytochrome P-450 Enzyme System / analysis*
  • Dogs
  • Female
  • Guinea Pigs
  • Hemoglobins / analysis
  • Kidney / enzymology*
  • Male
  • Mesocricetus
  • Mice
  • Microcomputers
  • Microsomes / enzymology*
  • Mitochondria / enzymology
  • Rabbits
  • Rats
  • Rats, Inbred Strains
  • Species Specificity
  • Spectrophotometry
  • Swine

Substances

  • Hemoglobins
  • Carbon Monoxide
  • Cytochrome P-450 Enzyme System