A Point Mutation Is Responsible for the Acquisition of Transforming Properties by the T24 Human Bladder Carcinoma Oncogene

Nature. 1982 Nov 11;300(5888):149-52. doi: 10.1038/300149a0.

Abstract

The genetic change that leads to the activation of the oncogene in T24 human bladder carcinoma cells is shown to be a single point mutation of guanosine into thymidine. This substitution results in the incorporation of valine instead of glycine as the twelfth amino acid residue of the T24 oncogene-encoded p21 protein. Thus, a single amino acid substitution appears to be sufficient to confer transforming properties on the gene product of the T24 human bladder carcinoma oncogene.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Line
  • Gene Expression Regulation
  • Genes, Viral
  • Humans
  • Mutation*
  • Neoplasm Proteins / genetics
  • Oncogenes*
  • Retroviridae / genetics
  • Urinary Bladder Neoplasms / genetics*

Substances

  • Neoplasm Proteins

Associated data

  • GENBANK/J00206
  • GENBANK/J00276
  • GENBANK/J00277
  • GENBANK/K00954