Identification of the Cap Binding Protein of Influenza Virus

Nucleic Acids Res. 1982 Aug 11;10(15):4803-12. doi: 10.1093/nar/10.15.4803.

Abstract

The presence of a cap binding protein in influenza virus PR8 has recently been demonstrated by photoaffinity labelling with the cap-analogue (gamma [3 2P]-[4-(benzoylphenyl)methylamido]-7-methylguanosine 5'-triphosphate). This paper describes the identification of the labelled protein using two-dimensional gel electrophoresis. The protein is shown to be PB2, the smaller of the two basic P proteins in the polymerase complex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carrier Proteins / genetics*
  • Cross-Linking Reagents*
  • DNA-Directed RNA Polymerases / metabolism
  • Kinetics
  • Orthomyxoviridae / genetics*
  • RNA Cap-Binding Proteins
  • Transcription, Genetic
  • Viral Proteins / genetics

Substances

  • Carrier Proteins
  • Cross-Linking Reagents
  • RNA Cap-Binding Proteins
  • Viral Proteins
  • DNA-Directed RNA Polymerases