Intrastrain recurrent idiotypes among anti-DNA antibodies of (NZB x NZW)F1 hybrid mice

Eur J Immunol. 1982 Sep;12(9):761-6. doi: 10.1002/eji.1830120911.


Immunization of NZB and A/J mice against an anti-DNA hybridoma antibody (F227) derived from (NZB x NZW)F1 (B/W) mice allowed the preparation of two anti-idiotypic antisera. These two reagents were shown to recognize different idiotopes of the F227 monoclonal antibody. NZB anti-idiotypic antibodies recognized non-ligand-modifiable idiotypic determinants. These idiotopes were private or present at undetectable level in BW mouse sera since it was found that only two of the 24 B/W mouse sera tested were recognized by these antibodies. Conversely, A/J anti-idiotypic antibodies recognized partially ligand-modifiable idiotopes which were found in all B/W mouse sera tested. These results demonstrate that anti-DNA antibodies share similar idiotypic specificities and suggest that these autoantibodies occur as families of structurally related proteins.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Autoantibodies / immunology*
  • DNA / immunology*
  • Hybridization, Genetic
  • Immunoglobulin G / immunology
  • Immunoglobulin Idiotypes*
  • Mice
  • Mice, Inbred NZB
  • Mice, Inbred Strains


  • Antibodies, Monoclonal
  • Autoantibodies
  • Immunoglobulin G
  • Immunoglobulin Idiotypes
  • DNA