The question of the nasotemporal overlap of ganglion cells in the central retina, which project to the ipsilateral and contralateral lateral geniculate nucleus, was reexamined in the cat by means of a retrograde fluorescent double-labeling technique. The horizontal separation between the ipsilateral and contralateral decussation lines, which corresponded to the width of the median strip of overlap, was approximately 0.2 mm. This is the first direct confirmation of the extent of such overlap in the cat retinal ganglion cells, which had been previously studied by indirect methods.