Species differences in the metabolic C- and N-oxidation , and N-methylation of [14C]pyridine in vivo

Xenobiotica. 1982 Aug;12(8):527-34. doi: 10.3109/00498258209038931.


1. The metabolism of [2,6-14C]pyridine in vivo has been investigated in the rat, hamster, mouse, gerbil, rabbit, guinea-pig, cat and man, and the quantitative determination of the various urinary metabolites carried out by radiochromatographic analysis. 2. Unchanged pyridine and its N-methylated metabolite, N-methylpyridinium ion, were determined using a Partisil-10 SCX cation-exchange h.p.l.c. column, whereas the C- and N-oxidation products were assayed by reverse-phase chromatography, using a Partisil-10 ODS column. 3. Most of the species studied produce pyridine N-oxide, N-methylpyridinium ion, 2-pyridone, 3-hydroxypyridine and 4-pyridone as metabolites, but the proportion of the dose excreted as each of these metabolites is species-dependent.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cats
  • Chromatography, Gas / methods
  • Chromatography, High Pressure Liquid / methods
  • Chromatography, Thin Layer / methods
  • Cricetinae
  • Female
  • Gerbillinae
  • Guinea Pigs
  • Humans
  • Male
  • Mesocricetus
  • Methylation
  • Mice
  • Oxidation-Reduction
  • Pyridines / metabolism*
  • Rabbits
  • Rats
  • Rats, Inbred Strains
  • Species Specificity


  • Pyridines
  • pyridine