Solvent systems for improved isolation and separation of territrems A and B

Appl Environ Microbiol. 1982 Oct;44(4):860-3. doi: 10.1128/aem.44.4.860-863.1982.

Abstract

Territrems A and B, tremorgenic mycotoxins in the rice culture of Aspergillus terreus, were extracted with hot chloroform. The toxins were cleaned on a silica gel column by direct adsorption-concentration of the extracts followed by desorption with chloroform-acetone (9:1, vol/vol). Crude toxin mixtures were separated by silica gel column chromatography and eluted with benzene-ethyl acetate (65:35, vol/vol). The method gave 112 mg of territrem A, 379 mg of territrem B, and 80 mg of their mixture from 4 kg of rice per batch. The criteria of extraction, cleanup, and separation are provided.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Thin Layer
  • Food Microbiology
  • Mycotoxins / isolation & purification*
  • Oryza / analysis*
  • Pyrans*
  • Solvents

Substances

  • Mycotoxins
  • Pyrans
  • Solvents
  • territrem A
  • territrem B