The interaction of DMSO-induced murine Friend leukaemia erythroblasts with cultured syngeneic mouse peritoneal macrophages: an in vitro model for the study of ineffective erythropoiesis

Br J Exp Pathol. 1982 Dec;63(6):586-93.

Abstract

The interaction between dimethyl sulphoxide-induced Friend leukaemia erythroblasts (IFLE) and cultured syngeneic mouse peritoneal macrophages (PM phi) was assessed radiochemically as well as by light and electron microscopy. The data revealed that a small number of the IFLE either became attached to or were ingested by the PM phi and that the interacting IFLE were usually well differentiated. Interactions of a similar nature were also seen when IFLE were co-cultured with macrophages derived from mouse bone marrow. The extent of interaction between IFLE and PM phi provides a useful system in which mechanisms underlying ineffective erythropoiesis can be defined.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells
  • Cell Communication
  • Cell Count
  • Cell Differentiation
  • Cells, Cultured
  • Cricetinae
  • Dimethyl Sulfoxide
  • Erythroblasts / physiology*
  • Erythrocytes / physiology*
  • Erythropoiesis*
  • Female
  • Leukemia, Experimental / chemically induced
  • Leukemia, Experimental / physiopathology*
  • Macrophages / physiology*
  • Mice
  • Mice, Inbred DBA
  • Microscopy, Electron

Substances

  • Dimethyl Sulfoxide