Separation and quantitation of cis- and trans-thiothixene in human plasma by high-performance liquid chromatography

J Chromatogr. 1982 Dec 10:233:257-67. doi: 10.1016/s0378-4347(00)81752-1.

Abstract

A high-performance liquid chromatographic procedure is described for the separation of cis and trans isomers of thiothixene, a thioxanthene derivative used as an antipsychotic agent. A radial compression module (RCM-100) was used with both silica and cyanopropyl cartridges. A fixed-wavelength UV detector (254 nm) was used in these studies for quantitation. Mesoridazine is used as an internal standard because of its separation characteristics and reproducible quantitation. C18 Sep-Pak cartridges are used for biological sample clean-up. Plasma samples from patients treated with thiothixene (Navane) were assayed for cis and trans-thiothixene. No trans-thiothixene was detectable and cis-thiothixene concentrations ranged from 0 to 22.5 ng/ml.

MeSH terms

  • Chromatography, High Pressure Liquid / methods
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Isomerism
  • Mesoridazine
  • Reference Standards
  • Thiothixene / blood
  • Thiothixene / isolation & purification*

Substances

  • Mesoridazine
  • Thiothixene