The immunological response of pigs and guinea pigs to antigens of African swine fever virus

Arch Virol. 1982;74(2-3):91-100. doi: 10.1007/BF01314703.


Pigs vaccinated with glutaraldehyde-fixed alveolar macrophages (AM) infected with African swine fever virus (ASFV) had an accelerated serological response after subsequent challenge and a slight reduction in levels of viraemia. Vaccination of pigs with detergent-treated infected AM produced no detectable serological response and no protection against homologous challenge. Guinea pigs were vaccinated with glutaraldehyde-fixed ASFV-infected cells, detergent-treated infected cells, detergent-treated infected spleen homogenate, purified ASFV or sonicated infected cells. Antibody was detectable by ELISA after vaccination with all preparations except the detergent-treated infected spleen vaccine. However, vaccination with purified ASFV or sonicated infected cells induced antibodies that were also strongly reactive in antibody-dependent cell-mediated cytotoxicity and complement-mediated lysis assays. If such antibodies are protective, immunization of pigs with purified ASFV or sonicated infected cells may induce a protective immunity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • African Swine Fever / immunology*
  • African Swine Fever Virus / immunology*
  • Animals
  • Antibodies, Viral / analysis
  • Antibodies, Viral / biosynthesis*
  • Antibody-Dependent Cell Cytotoxicity
  • Antigens, Viral / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Guinea Pigs
  • Iridoviridae / immunology*
  • Macrophages / microbiology
  • Neutralization Tests
  • Spleen / microbiology
  • Swine
  • Vaccination*
  • Viremia


  • Antibodies, Viral
  • Antigens, Viral