A new sensitive radioimmunoassay for plasma arginine vasopressin

J Immunoassay. 1982;3(2):115-31. doi: 10.1080/15321818208056990.


A new extremely sensitive radioimmunoassay to measure plasma AVP has been developed. Antiserum to AVP of high affinity (Keq = 1.1 X 10(12) 1/mol), raised in rabbits, showed little cross-reaction with related analogues (LVP less than 0.03%, AVT, DDAVP, OXT less than 0.01). The specific activity of 125I-AVP (chloramine T method) was 1400-1750 Ci/mmol. The limit of detection of plasma AVP, after extraction of 2 ml plasma with Florisil, was 0.3 pmol/1. Coefficient of variations of plasma control (2 pmo1/1) were 9.7% (intraassay) and 15.3% (inter-assay), n = 15. Osmotic stimulation by hypertonic saline infusion caused a linear response in plasma AVP in normal subjects, but plasma AVP remained undetectable in patients with cranial diabetes insipidus. Suppression of AVP secretion by a standard oral water load and by alcohol and fluid in volunteers produced low or undetectable values of plasma AVP. In a patient with the syndrome of inappropriate antidiuresis, plasma AVP concentration was grossly elevated.

MeSH terms

  • Animals
  • Arginine Vasopressin / blood*
  • Beer
  • Diabetes Insipidus / blood
  • Female
  • Humans
  • Immune Sera
  • Microchemistry
  • Rabbits
  • Radioimmunoassay


  • Immune Sera
  • Arginine Vasopressin