[Examination of protein digestibility in individual intestinal segments under in vivo conditions in experimental rats by the isotope dilution method]

Arch Tierernahr. 1982 Dec;32(12):841-52. doi: 10.1080/17450398209421033.
[Article in German]

Abstract

Test rats were labelled with 15N over a period of 7 days and killed on the 12th day. 220 min. before they were killed the diet was changed (wheat diet leads to whole egg diet) and they were given a 14C-leucine injection. The 15N-labelling of the digesta proved to be suitable for the determination of the digestibility of the feed proteins in the individual sections of the intestines. If the atom-% 15N-excess of the TCA-soluble fraction of the digesta is = 100%, the resulting digestibility of the protein fraction (TCA-precipitable fraction) corresponds to the 15N-dilution of the unlabelled feed protein. The following digestibility values were ascertained: (formula; see text) The calculation method suggested here cannot be applied to the large intestine because microbial activity influences the quotients. 14C-labelling was also insufficient for gathering statements on the digestibility of proteins.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Dietary Proteins / administration & dosage
  • Dietary Proteins / metabolism*
  • Digestion
  • Egg Proteins / metabolism
  • Intestine, Small / anatomy & histology
  • Intestine, Small / physiology*
  • Leucine / administration & dosage
  • Male
  • Plant Proteins / metabolism
  • Rats
  • Rats, Inbred Strains
  • Triticum

Substances

  • Dietary Proteins
  • Egg Proteins
  • Plant Proteins
  • Leucine