Mechanisms of p-aminohippurate transport by brush-border and basolateral membrane vesicles isolated from rat kidney cortex

Biochim Biophys Acta. 1982 Oct 22;692(1):97-100. doi: 10.1016/0005-2736(82)90506-5.


The uptake of [3H]-labeled p-aminohippurate by brush-border and basolateral membrane vesicles isolated from rat renal cortex has been studied by a rapid filtration technique. Some characteristics of carrier-mediated transport for p-aminohippurate were demonstrated in basolateral membrane vesicles: the uptake was inhibited by probenecid or 4,4'-diisothiocyano-2,2'-disulfonic stilbene (DIDS), was saturable, was stimulated by the countertransport effect, and showed discontinuity in the Arrhenius plot. In contrast, brush-border membrane vesicles failed to display saturability of p-aminohippurate uptake and stimulation by the countertransport effect, although probenecid and DIDS reduced the uptake. Furthermore, p-aminohippurate uptake by brush-border membrane vesicles was influenced more sensitively by alteration in the membrane potential compared with that by basolateral membrane vesicles.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
  • 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / analogs & derivatives
  • 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / pharmacology
  • Aminohippuric Acids / metabolism*
  • Animals
  • Biological Transport, Active / drug effects
  • Cell Membrane / metabolism
  • In Vitro Techniques
  • Kidney Cortex / metabolism*
  • Kinetics
  • Male
  • Membrane Potentials
  • Microvilli / metabolism
  • Probenecid / pharmacology
  • Rats
  • Rats, Inbred Strains
  • p-Aminohippuric Acid / metabolism*


  • Aminohippuric Acids
  • 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
  • Probenecid
  • 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
  • p-Aminohippuric Acid