Double minute chromosomes and the homogeneously staining regions in chromosomes of a human neuroblastoma cell line

Science. 1977 Nov 18;198(4318):739-41. doi: 10.1126/science.71759.


Four human neuroblastoma cell lines were studied by chromosome banding techniques. All of the lines contained a marker chromosome with a long nonbanding homogeneously staining region (HSR). The HSR-containing chromosome differed in each line. One line contained two classes of cells: one with an HST marker chromosome and the other with double minute chromosomes. Each cell had one of these abnormalities; no cell had both. The presence of two additional chromosomal markers in all cells of this line indicates a common origin. These observations suggest that the double minute chromosomes are derived from the HSR.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line
  • Chromosome Aberrations*
  • Chromosomes, Human / ultrastructure*
  • Humans
  • Karyotyping
  • Neuroblastoma / ultrastructure*
  • Staining and Labeling