Immunogenicity of leptospiral vaccines grown in protein-free medium

J Med Microbiol. 1982 Nov;15(4):493-501. doi: 10.1099/00222615-15-4-493.


Leptospira interrogans serovars pomona and hardjo were adapted to grow in a chemically-defined, protein-free (PF) medium. Formolised monovalent vaccines of serovars pomona and hardjo and a bivalent mixture of the two were prepared from PF cultures. Live PF cultures and the vaccine preparations retained their agglutinating antigens and their immunogenicity when tested in rabbits and guinea-pigs. The vaccines were not pyrogenic and dermal reactions were slight.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agglutinins / analysis
  • Animals
  • Bacterial Vaccines / immunology*
  • Culture Media
  • Formaldehyde
  • Guinea Pigs
  • Leptospira / immunology*
  • Rabbits
  • Skin Tests


  • Agglutinins
  • Bacterial Vaccines
  • Culture Media
  • Formaldehyde