Rapid synthesis of oligodeoxyribonucleotides. VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide support

Nucleic Acids Res. 1982 Oct 25;10(20):6243-54. doi: 10.1093/nar/10.20.6243.

Abstract

A new kieselguhr-polydimethylacrylamide support has been used in a continuous flow, column system for solid phase synthesis of oligodeoxyribonucleotides by a phosphotriester procedure. Using only protected mononucleotides a 14-mer, 20-mer and 27-mer were assembled in high repetitive yield using a simple manually operated, bench top apparatus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylamides
  • Base Sequence
  • Chromatography, High Pressure Liquid
  • Chromatography, Ion Exchange
  • Deoxyribonucleotides
  • Diatomaceous Earth
  • Indicators and Reagents
  • Methylmethacrylates
  • Oligodeoxyribonucleotides / chemical synthesis*
  • Oligonucleotides / chemical synthesis*

Substances

  • Acrylamides
  • Deoxyribonucleotides
  • Indicators and Reagents
  • Methylmethacrylates
  • Oligodeoxyribonucleotides
  • Oligonucleotides
  • Diatomaceous Earth