Mapping a marsupial X chromosome using kangaroo-mouse somatic cell hybrids

Cytogenet Cell Genet. 1981;29(3):127-37. doi: 10.1159/000131561.

Abstract

A series of marsupial-eutherian somatic cell hybrids was produced by fusion between lymphocytes from the red kangaroo (Macropus rufus) and HPRT-deficient mouse cells. The hybrids lost marsupial chromosomes and could therefore be used to map marsupial genes. Several of the hybrids contained a complete red kangaroo X chromosome and expressed the kangaroo form of the enzymes HPRT, G6PD, and PGKA. A number of HPRT-deficient revertant cell lines were derived from the hybrids. These possessed a variety of partially deleted X chromosomes. With these cell lines, it has been possible to establish the X-linkage of the genes for HPRT, G6PD, and PGKA in this marsupial and to localize these three genes to the terminal portion of the euchromatic arm of the red kangaroo X chromosome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Chromosome Banding
  • Chromosome Mapping*
  • Female
  • Glucosephosphate Dehydrogenase / genetics
  • Hybrid Cells
  • Hypoxanthine Phosphoribosyltransferase / genetics
  • Macropodidae / genetics*
  • Macropodidae / metabolism
  • Marsupialia / genetics*
  • Mice
  • Phosphoglycerate Kinase / genetics
  • Sex Chromosomes*
  • X Chromosome*

Substances

  • Glucosephosphate Dehydrogenase
  • Hypoxanthine Phosphoribosyltransferase
  • Phosphoglycerate Kinase