Abstract
N-Methyl mesoporphyrin was a powerful inhibitor of protohaem ferro-lyase in vitro, whereas N-ethyl mesoporphyrin and N-methyl coproporphyrin were not and neither was the newly described green pigment produced by giving rats ethylene. This suggests that the size of the substituent at a pyrrole nitrogen and also the number of carboxylic acid side chains of the substituted porphyrin are important for the inhibitory effect. Evidence that N-methyl mesoporphyrin inhibited the enzyme, whereas the ethylene-derived pigment did not, was also obtained in vivo.
MeSH terms
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5-Aminolevulinate Synthetase / metabolism
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Animals
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Coproporphyrins / chemical synthesis
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Coproporphyrins / metabolism
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Coproporphyrins / pharmacology*
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Ferrochelatase / antagonists & inhibitors*
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Liver / metabolism
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Lyases / antagonists & inhibitors*
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Male
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Mesoporphyrins / chemical synthesis
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Mesoporphyrins / metabolism
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Mesoporphyrins / pharmacology*
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Mice
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Pigments, Biological / pharmacology
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Porphyrins / pharmacology*
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Spectrophotometry
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Structure-Activity Relationship
Substances
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Coproporphyrins
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Mesoporphyrins
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Pigments, Biological
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Porphyrins
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N-ethyl mesoporphyrin
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N-methyl coproporphyrin
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5-Aminolevulinate Synthetase
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Lyases
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Ferrochelatase