High-performance liquid chromatographic procedure for the simultaneous determination of the natural polyamines and their monoacetyl derivatives

J Chromatogr. 1980 Dec 12;221(2):227-35. doi: 10.1016/s0378-4347(00)84307-8.

Abstract

The separation of the natural polyamines and their monoacetyl derivatives by high-performance reversed-phase liquid chromatography is reported. Octane sulfonate was used to form ion pairs with the polycations and acetylspermidine determinations directly from tissue extracts and body fluids without pre-purification.

MeSH terms

  • Animals
  • Chromatography, High Pressure Liquid / methods*
  • Humans
  • Liver / analysis
  • Male
  • Octanes
  • Polyamines / analysis*
  • Polyamines / urine
  • Rats
  • Spermidine / analogs & derivatives*
  • Spermidine / analysis
  • o-Phthalaldehyde

Substances

  • Octanes
  • Polyamines
  • N(1)-acetylspermidine
  • o-Phthalaldehyde
  • Spermidine
  • octane