A rapid one-step procedure for purification of mononuclear and polymorphonuclear leukocytes from human blood using a modification of the Hypaque-Ficoll technique

J Immunol Methods. 1978;24(3-4):389-93. doi: 10.1016/0022-1759(78)90143-6.

Abstract

A one-step procedure for purification of mononuclear and polymorphonuclear cells from human blood is described. It is a modification of the Hypaque-Ficoll method with density of 1.095 g/ml. Centrifugation at 200 X g for 20--30 min resulted in the separation of mononuclear and polymorphonuclear cells into 2 distinct bands at the interface. The mononuclear cell fraction contained 83.9 +/- 1.6% lymphocytes and 13.8 +/- 2.3% monocytes, while the other conssisted of highly purified neutrophils (96.4 +/- 1.0%). Leukocyte recovery by this method was always greater than 80% and viability exceeded 98%. Both cell fractions retained their immunological functions.

MeSH terms

  • Blood
  • Centrifugation, Density Gradient / methods*
  • Ficoll
  • Humans
  • Leukocytes*
  • Lymphocytes / physiology
  • Neutrophils* / physiology

Substances

  • Ficoll