Abstract
Submitochondrial particles from beef heart mitochondria synthesize ADP-arsenate from ADP and arsenate when energized by succinate. The ADP-arsenate formed hydrolyzes rapidly and this is almost certainly the mechanism by which arsenate "uncouples" oxidative phosphorylation. When sufficient hexokinase is present, a substantial portion of the ADP-arsenate formed reacts with glucose to form glucose 6-arsenate and ADP. The glucose 6-arsenate thus formed hydrolyzes, at pH 7.5 and 30 degrees C, under the conditions used, with a rate constant of 5.5 X 10(-4) s-1 and is a substrate for glucose-6-phosphate dehydrogenase.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Diphosphate / metabolism*
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Adenosine Triphosphate / metabolism
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Animals
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Arsenates / metabolism*
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Arsenic / metabolism*
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Cattle
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Glucose / metabolism
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Glucosephosphate Dehydrogenase / metabolism
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Hexokinase / metabolism
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Mitochondria / metabolism*
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NADP / metabolism
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Phosphates / metabolism
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Submitochondrial Particles / drug effects
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Submitochondrial Particles / metabolism*
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Succinates / pharmacology
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Succinic Acid
Substances
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Arsenates
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Phosphates
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Succinates
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NADP
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Adenosine Diphosphate
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Adenosine Triphosphate
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Succinic Acid
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Glucosephosphate Dehydrogenase
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Hexokinase
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Glucose
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Arsenic
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arsenic acid