The effect of iron and transferrin on the response of serum-free cultures of mouse lymphocytes to concanavalin A and lipopolysaccharide

Immunology. 1981 Jun;43(2):387-92.

Abstract

Incorporation of [14C]-thymidine into mouse lymph node cells stimulated with either Con-canavalin A or lipopolysaccharide in serum-free medium was markedly enhanced by the addition of transferrin. Thymidine incorporation was similar in transferrin-containing serum-free medium and in medium containing 5% foetal calf serum. Transferrins from both homologous and heterologous species were equally effective, but iron-binding half-molecules of transferrin, and low molecular weight iron chelates produced no enhancement. The optimal response was obtained with 10--50 micrograms/ml of transferrin, and with 30%--70% iron saturation. Although the major function of transferrin in lymphocyte cultures is probably to supply iron, it may also fulfil other functions.

MeSH terms

  • Animals
  • Cells, Cultured
  • Concanavalin A / pharmacology
  • Iron / pharmacology*
  • Iron Chelating Agents / pharmacology
  • Lipopolysaccharides / pharmacology
  • Lymphocyte Activation / drug effects*
  • Lymphocytes / metabolism
  • Mice
  • Mice, Inbred C3H
  • Molecular Weight
  • Thymidine / metabolism
  • Transferrin / pharmacology*

Substances

  • Iron Chelating Agents
  • Lipopolysaccharides
  • Transferrin
  • Concanavalin A
  • Iron
  • Thymidine