Streptomyces setonii: catabolism of vanillic acid via guaiacol and catechol

Can J Microbiol. 1981 Jun;27(6):636-8. doi: 10.1139/m81-097.

Abstract

Streptomyces setonii (strain 75Vi2) was grown at 45 degrees C in liquid media containing simple aromatic compounds as principal carbon sources. Thin-layer chromatography, UV spectrophotometry, and gas chromatography were used to show that S. setonii converted benzoic acid, guaiacol, and vanillic acid to catechol; p-hydroxybenzoic acid to protocatechuic acid; and m-hydroxybenzoic acid to gentisic acid. Presence of the ring-cleavage enzymes catechol 1,2-dioxygenase, protocatechuate 3,4-dioxygenase, and gentisate 2,3-dioxygenase was shown both by O2 uptake in ring-cleavage reactions catalyzed by cell-free extracts and by changes in UV spectra that indicated the presence of specific ring-cleavage products. A unique feature of this strain was its catabolism of vanillic acid by of guaiacol and catechol, using a pathway that had not been confirmed previously.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Benzoates / metabolism
  • Catechol 1,2-Dioxygenase
  • Catechols / metabolism
  • Dioxygenases*
  • Guaiacol / metabolism
  • Hydroxybenzoates / metabolism*
  • Oxygenases / metabolism
  • Protocatechuate-3,4-Dioxygenase / metabolism
  • Streptomyces / metabolism*
  • Vanillic Acid / metabolism*

Substances

  • Benzoates
  • Catechols
  • Hydroxybenzoates
  • Guaiacol
  • Oxygenases
  • Dioxygenases
  • Catechol 1,2-Dioxygenase
  • Protocatechuate-3,4-Dioxygenase
  • gentisate 1,2-dioxygenase
  • Vanillic Acid