The amino acid sequence of the gamma-subunit of mouse submaxillary gland 7 S nerve growth factor

J Biol Chem. 1981 Sep 10;256(17):9156-66.

Abstract

The complete amino acid sequence of the gamma-subunit of mouse submaxillary gland 7 S nerve growth factor has been determined from analyses of the peptides generated by cyanogen bromide, trypsin, and chymotrypsin from the naturally occurring fragments. All peptides were sequenced automatically in a spinning-cup sequenator using Polybrene to minimize extractive losses by the solvents employed thoughout the degradation cycles. The gamma-subunit, a serine protease with arginine specificity, contains 233 amino acid residues and shares sequence homology with other proteases of this family. The five disulfide bonds of the gamma-subunit are a subset of the six disulfides present in bovine trypsin, as judged by the location of the half-cystine residues in the primary structure. An N-linked carbohydrate side chain is attached to Asn-78 in at least a majority of tee gamma-molecules.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Carboxypeptidase B
  • Carboxypeptidases
  • Cyanogen Bromide
  • Disulfides / analysis
  • Macromolecular Substances
  • Mice
  • Nerve Growth Factors*
  • Peptide Fragments
  • Protein Conformation
  • Submandibular Gland / analysis*

Substances

  • 7S nerve growth factor complex
  • Disulfides
  • Macromolecular Substances
  • Nerve Growth Factors
  • Peptide Fragments
  • Carboxypeptidases
  • Carboxypeptidase B
  • Cyanogen Bromide