Enzyme-linked immunosorbent assay for anti-histone antibodies and their presence in systemic lupus erythematosus sera

J Immunol Methods. 1981;44(3):311-22. doi: 10.1016/0022-1759(81)90049-1.


We describe an enzyme-linked immunosorbent assay (ELISA) with adsorption of histones (total and fractions) on glass beads and saturation of excess sites with sheep serum. The anti-histone antibodies are detected with peroxidase conjugate and developed with Trinder's reagent which has great stability. This very sensitive method detects anti-histone antibodies in 53% of SLE patients and in virtually no other diseases. Positive reactions are observed only with total histones and fractions H1 and H2b.

MeSH terms

  • Absorption
  • Animals
  • Antibodies*
  • Cattle
  • Enzyme-Linked Immunosorbent Assay
  • False Positive Reactions
  • Histones / immunology*
  • Humans
  • Hydrogen-Ion Concentration
  • Lupus Erythematosus, Systemic / immunology*
  • Osmolar Concentration
  • Sheep


  • Antibodies
  • Histones