Temporal pattern of cleavage and the onset of gastrulation in amphibian embryos developed from eggs with the reduced cytoplasm

J Embryol Exp Morphol. 1981 Apr;62:83-94.


Fertilized eggs of the Japanese newt, Cynops pyrrhogaster, were divided into two or four equal-sized parts with fine glass rods before the first cleavage. In such cases one of the egg fragments, at least, proceeds to cleavage and gastrulates. The temporal pattern of cell division and the onset of gastrulation in such half or quarter embryos were investigated and compared with normal development. The following results were obtained: (1) desynchronization starts two divisions earlier in quarter embryos and one division earlier in half embryos compared with whole embryos, (2) the time from the first cleavage to the onset of gastrulation does not widely vary among quarter, half and whole embryos and (3) the numbers of blastomeres which constitute embryos at the pigment stage decrease in proportion to the diminution of egg volume.

MeSH terms

  • Animals
  • Blastomeres / physiology
  • Cell Count
  • Cell Division
  • Cleavage Stage, Ovum / physiology*
  • Cytoplasm / physiology
  • Gastrula / physiology*
  • Salamandridae / embryology*
  • Time Factors