Glutathione-S-transferase of human red blood cells; assay, values in normal subjects and in two pathological circumstances: hyperbilirubinemia and impaired renal function

Clin Chim Acta. 1981 Dec 9;117(2):209-17. doi: 10.1016/0009-8981(81)90040-1.

Abstract

An assay for human erythrocyte glutathione-S-transferase is described. The procedure is both sensitive and reproducible; sampling and storage conditions are also investigated. Reference values are given for normal neonates, children and adults. The normal red cell glutathione-S-transferase activity decreases significantly during the first weeks of life and remains constant afterwards. A slight but significant increase is observed over 75 years. Sex difference has no influence on enzyme activity. A significant increase (up to fourfold) in red cell glutathione-S-transferase activity is noted in newborns with hyperbilirubinemia exceeding 135 mumol/l. Red cell glutathione-S-transferase is also significantly increased in hemodialysed subjects suffering from renal dysfunction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anticoagulants / pharmacology
  • Erythrocytes / enzymology*
  • Glutathione Transferase / blood*
  • Humans
  • Hyperbilirubinemia / enzymology*
  • Kidney Diseases / enzymology*
  • Renal Dialysis
  • Substrate Specificity

Substances

  • Anticoagulants
  • Glutathione Transferase