Tricyclic antidepressant determination in human plasma by gas-liquid chromatography using nitrogen-phosphorous detection: application to single-dose pharmacokinetic studies

Pharmacology. 1981;23(2):57-63. doi: 10.1159/000137528.


A method with adequate sensitivity (to 0.50-0.75 mg/ml) for single-dose pharmacokinetic studies which utilizes gas-liquid chromatography with nitrogen-phosphorous detection for tricyclic antidepressants (imipramine, desipramine, amitriptyline, nortriptyline, and doxepin) is described. A basic (pH 13) extraction with acid back-wash and subsequent basic reextraction is used for plasma sample preparation. Standard curves, using clomipramine as the internal standard, are linear for concentrations from 1 to 200 ng/ml for all tricyclic antidepressants. Applicability of the method is demonstrated by a pharmacokinetic study in a normal volunteer who received 12.5 mg imipramine hydrochloride i.v.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antidepressive Agents, Tricyclic / blood*
  • Chromatography, Gas / methods*
  • Humans
  • Kinetics
  • Nitrogen
  • Phosphorus
  • Reference Standards


  • Antidepressive Agents, Tricyclic
  • Phosphorus
  • Nitrogen