A multi-wavelength spectrophotometric method for the simultaneous determination of five haemoglobin derivatives

J Clin Chem Clin Biochem. 1981 Jul;19(7):457-63. doi: 10.1515/cclm.1981.19.7.457.

Abstract

A method is described by which the concentration of deoxyhaemoglobin, oxyhaemoglobin, carboxyhaemoglobin, haemoglobin and sulfhaemoglobin in a human blood sample is determined by passing the haemolysate without air contact through a coarse filter and subsequently measuring the absorbance at lambda = 500, 569, 577, 620 and 760 nm. The ensuing set of equations is solved by matrix calculation with the aid of a simple computer program. The method has been tested by comparing it with conventional methods for the determination of the various haemoglobin derivatives separately.

MeSH terms

  • Carboxyhemoglobin / analysis
  • Computers
  • Hemoglobins / analysis*
  • Humans
  • Mathematics
  • Oxyhemoglobins / analysis
  • Spectrophotometry / methods*
  • Sulfhemoglobin / analysis

Substances

  • Hemoglobins
  • Oxyhemoglobins
  • Sulfhemoglobin
  • Carboxyhemoglobin