Laboratory method for measuring small amounts of tissue glycogen

Biochem Exp Biol. 1980;16(2):175-9.

Abstract

A procedure is reported which is well applicable for the quantitative determination of glycogen, especially in cases of a small glycogen content in a small amount of tissue. The method consists of extraction of glycogen by boiling potassium hydroxide, precipitation with ethanol, hydrolysis with sulphuric acid, and determination of the glycogen in form of glucose obtained with orthotoluidine.

MeSH terms

  • Animals
  • Glycogen / analysis*
  • Ileum / analysis
  • Intestinal Mucosa / analysis
  • Methods
  • Rats
  • Time Factors

Substances

  • Glycogen